The Contribution of Thymidine Phosphorylase in THP-1 Cell Survival.
| Autor: | Chih-Chuan Wang, 王致權 |
|---|---|
| Rok vydání: | 2008 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 Thymidine phosphorylase (TP) catalyses the reversible phospholysis of thymidine. In addition to its role in nucleotide metabolism, TP is also an angiogenic factor. Its catabolic product, 2-deoxy-ribose produced by phospholysis of thymidine, induces directional migration of endothelial cells in vitro and angiogenesis in vivo. It has been shown that the expression of TP is elevated in solid tumor and tumor-associated macrophage. TP may promote tumor progression by inducing angiogenesis and inhibiting aopotosis of tumor cells. The expression level of TP in most normal tissues is very low. But only in monocytes, myeloid and dendritic cells, TP is highly expressed. In this study, I investigated the role of TP in monocytic leukemia cell line, THP-1. By silencing of TP expression, we found that TP knockdown induces growth retardation and cell death, indicating that TP is necessary for the proliferation and survival of THP-1 cells. However, the cell death induced by TP knockdown was not reversed by either thymidine, 2-deoxy-ribose, or thymine, suggesting its survival role other than its metabolic functions in dTTP synthesis. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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