The influence of various substrates and surface topographics on hepatocytic morphology and function
| Autor: | Ko-Liang Kuo, 郭科良 |
|---|---|
| Rok vydání: | 2008 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 The purpose of this study is to develop an in vitro drug efficiency screening system for replacing the traditional animal model. In this study, the patterned-PDMS was coated with various substrates, including collagen and pectin. Besides surface coating, we also immobilized galactose on the PDMS surface since galactose was well known as ligand for ASGPR (asialoglycoprotein receptor) of primary hepatocyte. Through the specific ligand-receptor binding, primary hepatocytes could display the spheroid morphology which could maintain the liver cellular specific functions. From the results of Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) and electron spectroscopy for chemical analysis (ESCA) indicated that we had galactosylated of the surfaces of PDMS successfully. Furthermore, we utilized primary hepatocyte and HepG2 cells, a human hepatocellular liver carcinoma cell line, to examine the effects of specific substratum for the cellular behaviors. From the results of F-actin and DAPI staining, we found that HepG2 cells displayed circular morphology and cell-aggregates on pectin-coated and galactosylated PDMS, but expressed a spreading phenomenon on collagen-coated PDMS. Moreover, the result of EROD assay proved that galactosylated PDMS could maintain the CYP1A1/2 activity of primary hepatocyte more than one week. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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