Preparation and Application of Surface Acoustic Wave Gas- and Bio-Sensor
| Autor: | Hung Wei, Chang, 張宏維 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 Surface acoustic wave gas and Bio-sensors were developed to detect organic gases and biospecies, e.g. proteins and insulin. An inexpensive surface acoustic wave (SAW) sensor system was developed and used to detect trace pollutants in the air. For liquid environment, a shear-horizontal surface acoustic wave sensor system was established to apply in immunosensor system to detect various samples, e.g. hemoglobin, myoglobin, gliadin, and insulin, in aqueous solution. The homemade SAW gas sensor is composed of a low cost (< 10 $USD) SAW chip for correspondence, an inexpensive counter and RS-232 computer interface with a written computer program for frequency signal acquisition and data processing. With different adsorbent coatings, various SAW gas sensors were prepared to detect organic pollutants, e.g. carboxylic acids, aldehydes, alchohols, alkanes, alkenes, alkynes in the air. Furthermore, the multi-channel SAW gas detection system was also developed to detect various air pollutants simultaneously. Shear horizontal surface acoustic wave sensors immobilized with C60/proteins, e.g. C60/hemoglobin(C60-Hb), C60/myoglobin(C60-Mb), and C60/gliadin, and C60/anti-insulin coatings were prepared and applied to detect specific antibodies, e.g. anti-hemoglobin, anti-myoglobin, anti-gliadin, insulin, respectively, in liquid environments. The immobilizations of hemoglobin and myoglobin onto fullerene were studied through a C60-coated SH-SAW sensor system in liquid. The partially irreversible responses for these proteins were observed by the desorption study, which implied that fullerene could chemically react with these proteins. Both C60-Hb and C60-Mb coating materials were successfully prepared and identified with an FTIR spectrometer. The C60-Hb and C60-Mb coated SH-SAW immunosensors exhibited linear frequency responses to the concentration of anti-Hb and anti-Mb antibodies with sensitivities of 0.14 and 1.27 kHz/ (g/mL), respectively. Both C60-protein coated SH-SAW immunosensorms showed detection limits of 0.32 and 0.035 g/mL for anti-Hb and anti-Mb antibodies, respectively, in aqueous solution. In addition, dual channel SAW immunosensors coated with C60-Hb and C60-Mb were prepared and applied to detect anti-Hb and anti-Mb antibodies simultaneously. An immobilized fullerene C60/anti-insulin antibody was prepared and applied in shear horizontal surface acoustic wave (SH-SAW) immunosensors to detect insulin in aqueous solutions. Within the range of normal human insulin concentration, the SH-SAW immunosensors immobilized with C60/anti-insulin coating exhibited linear frequency responses to the concentration of insulin with sensitivity of 130 Hz/pM. The SH-SAW immunosensor with C60/anti-insulin coating showed detection limit of 0.58 pM for insulin in aqueous solution. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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