Designed and Validated Cone and Plate Bioreactor
| Autor: | Wen-fu Wang, 王文甫 |
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| Rok vydání: | 2008 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 The progress and development of medical science has an inseparable relation with technology. The new frontier in latest development - tissue engineering, is a technology using cell-scaffold constructs developed in vitro to replace injury or function losing organs and tissues. Bioreactors are an indispensable device for the development of tissue engineering. They provide not only basic living environments for cells, but also mock up the environments for functional tissues to form. Besides, bioreactors may also be used to apply various different mechanical stimuli to control cells’ gene expression or differential pathway. In this study, a bioreactor which can be used to culture cells in vitro and provide quantitative shear stress has been designed. The model of the design is based on the regulation of the cone and plate viscometer, and the stress values are computed using the experiential formula proposed in previous literature. The in-house bioreactor was used to provide four hours of shear stress in a day in 0, 0.8, 1.2, 1.6 Pa respectively to stimulate the rat bone marrow stem cells. Results show when different shear stresses were applied, the cell shape and proliferation rate were not changing obviously comparing to the case of no shear imposed. This study demonstrates that the bioreactor constructed in house can be a stable device providing broaden shear stresses. With this devices, further tests on the cell responses to the shear stimulation can be conducted for tissue engineering applications. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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