A study on the feasibility of using positively charged catanionic vesicles as DNA carriers
| Autor: | Chih-Jung Wu, 吳芷容 |
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| Rok vydání: | 2008 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 This study investigated the formation behavior of positively charged catanionic vesicles and the association behavior of the catanionic vesicles with DNA by the vesicle size and zeta potential analyses. The transfection characteristics of the catanionic vesicle/DNA complexes were analyzed further. An ion pair amphiphile (IPA), hexadecyltrimethylammonium- dodecylsulfate (HTMA-DS), was prepared first by mixing the cationic surfactant, hexadecyltrimethylammonium bromide (HTMAB), and anionic surfactant, sodium dodecylsulfate (SDS), in water. The HTMA-DS was then used as the main material with the addition of various molar ratios of dialkyldimethylammonium bromides to form positively charged catanionic vesicles by an appropriate process. The vesicle size analysis indicated that the physical stability of the catanionic vesicles was significantly deteriorated when the vesicle dispersions were diluted, and could be enhanced with the presence of a proper amount of cholesterol. In addition, the charge characteristic of the catanionic vesicles in a buffer solution became less significant. However, by adjusting the composition of the IPA/cationic surfactant mixtures, catanionic vesicles with acceptable physical stability and charge characteristic could be prepared. By analyzing the sizes and zeta potentials of the catanionic vesicle/DNA complexes with various compositions, it was found that the positively charged catanionic vesicles could associate with DNA. The transfection experiments performed with the catanionic vesicle/DNA complexes indicated that the transfection efficiency varied with the types of cells. For U937 cells, the transfection efficiency was negligible. However, if the Hela cells were used in the transfection experiments, the transfection efficiency of the catanionic vesicles could be 80% of that of a commercial transfection reagent. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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