Separation of bioactive compounds from Curcuma longa L. and Pelodiscus sinensis by using supercritical carbon dioxide
| Autor: | Li-Hsun Chang, 張立勳 |
|---|---|
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 This study investigated the extraction of bioactive compounds from nature products by using supercritical carbon dioxide (SC-CO2). Experiments were performed for two materials, one is Curcuma longa L. (turmeric) and the other is Pelodiscus sinensis (soft-shelled turtle fish, SST). 7 wt% of turmeric oil yield containing 71 wt% purity of turmerones was obtained from fresh turmeric by using SC-CO2 extraction at 314 K and 25 MPa within 2.5 hr. Three major turmerones were purified from the SC-CO2 extractive turmeric oil by chromatographing in a normal phase silica gel 60 column. 86 wt% purity of ar-turmerone and 81 wt% purity of α+β-turmerone have been obtained. In vitro, this SC-CO2 extractive turmeric oil inhibited the growth of lung cancer cell (A549), showing IC50 of 160 μg/mL, and 50 % of phogocytosis activity in rat macrophage (Raw 264.7) was also inhibited by 4μg/mL of this extracted oil. Following SC-CO2 deoiled process of turmeric material, the residue of SC-CO2 extracted turmeric contains 10 wt% of curcumins and other active compounds of activating alcohol dehydrogenase (ADH). These bioavailable compounds were obtained from this SC-CO2 extracted turmeric by using an ethanol solution ultrasonicated at 313 K for 240 min. 89 % of curcumins was successfully recovered and 53 wt% purity of curcumins was obtained. The ADH activity in rat hepatocytes treated with these extracts of deoiled turmerics has been assayed in vitro. Our experimental results indicated that the extracts of fresh turmeric and SC-CO2 deoiled turmeric increased the ADH enhancement factor by 16 % and 35 %, respectively. This bioavailability investigation clearly evidenced that the enhancing ADH activity in rat hepatocytes was mainly attributed to the content of curcummins in the extract. Finally, this work studied the recovery of omega-3 fatty acids from SST oil bags. 53 wt% crude oil yield was obtained by using pressurized carbon dioxide with top-down fluid flow at 55 bar and 328 K. 81 wt% of C20-24 fatty acid was then enriched using a counter-current SC-CO2 extractive distillation from an esterified oil mixture at 353 K and 250 bar. The column-height versus concentration of C20-24 was examined using the Riccati equation. This refined oil promoted the catalase release of rat macrophage, showing 110 mole/min/mL of catalase activity. The results indicated that the anti-oxidant activity of SST oil was enhanced after SC-CO2 concentration process. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|