The antioxidant activity and neuroprotective effects of Desmodium genus and related plants in Taiwan
| Autor: | Min-Chung Hsueh, 薛閔鐘 |
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| Rok vydání: | 2008 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 96 Parkinson’s disease, one of widespread neurodegenerative disorders, is related to intercellular oxidative stress caused by overproduction of free radical. Therefore, recent researches indicated that MAO-B inhibitor and antioxidants may prevent Parkinson’s disease. This study was aimed to investigate the antioxidant components, anti-oxidative activity and MAO inhibiting effects of four specise of Desmodium plants and three related plants by microtiter spectrophotometric and fluorescent methods. First, the experimental results demonstrated that Phyllodium pulchellum, Desmodium sequax and Codariocalyx motorius have enriched antioxidant components including total phenol, flavonoid, and total phenylpropanoid glycoside. These above three plants also have the better antioxidative capacity than the others. There are a very closely correlation among total phenols content, Trolox equivalent antioxidant capacity and reducing power of the selected seven plants. Second, Phyllodium pulchellum, Desmodium sequax and Codariocalyx motorius have the better superoxide anion radical scavenging activity. The Codariocalyx motorius and Tadehagi triquetrum have better hydrogen peroxide and hydroxyl radical scavenging activity than the others. On the other hand, Codariocalyx motorius and Desmodium sequax have better MAO-A and MAO-B inhibiting effects, and Desmodium sequax has better selectivity in inhibiting MAO-bactivity. Furthermore, Phyllodium pulchellum, Desmodium sequax, Codariocalyx motorius, Tadehagi triquetrum, possessing the better antioxidant potency were selected to compare the neuroprotective effects on 6-OHDA induced oxidative damage in SH-SY5Y neuroblastoma cell. The present result demonstrated that Phyllodium pulchellum, Codariocalyx motorius, and Tadehagi triquetrum can elevate cell viability, but Desmodium sequax can not. Phyllodium pulchellum, Codariocalyx motorius, and Tadehagi triquetrum protected the 6-OHDA-induced oxidative damage in SH-SY5Y neuroblastoma cell via decreasing intracellular oxidative stress and have better antioxidative. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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