Interactions between Submicron Polymer Particles and Binary Proteins
| Autor: | Yu-ting Weng, 翁郁婷 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Submicron polymer particles were prepared by emulsion polymerization with methyl methacrylate (MMA) as the hydrophobic monomer, sodium persulfate as the initiator, and HS-10 as the surfactant. These particle surfaces have sulfate groups (-SO4-) and sulfonate groups (-SO3-) at the same time. Therefore, they are relatively insensitive to changes in pH. The adsorption of binary proteins (BSA and lysozyme) on the particles was studied. Isothermal adsorption of binary proteins onto the particles leads to changes in the zeta potential, particle size and turbidity. At the low ionic strength, the total maximum protein adsorption occurs in the pH range of 7－8. This may well be due to lysozyme adsorption onto the particles first and this will result in increased net negative charge particles and therefore promote the adsorption of BSA. Another reason is that BSA and lysozyme have opposite net charges, they’ll aggregate with each other first by electrostatic attraction forces. Then electrostatic attraction forces between lysozyme of the binary proteins mixture and submicron latex particles bring about the enhanced total adsorbed amount. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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