Multifunctional Silica Nanoparticles in Biological Application
| Autor: | Si-Han Wu, 吳思翰 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Mesoporous silica nanoparticles (MSN) with unique properties such as high surface area, uniform pore size, easy modification, and biocompatibility have made them suitable for biological applications. In previous reports, MSNs have been demonstrated to be cell markers, gene transfection and drug delivery agents. Although these cell-level studies are attractive, some important issues, such as the cellular uptake efficiency, toxicity and circulation behavior of MSN in living animals, have to be addressed for further practical animal-level applications. Superparamagnetic nanoparticles (i.e., magnetite) with diameter less than 25 nm exhibit an effective magnetic resonance imaging (MRI) contrast enhancement behavior. Because MRI is a noninvasive imaging method, it is a powerful tool to track the migration of cells and to investigate the distribution of nanoparticles in living body. But the main drawbacks of MRI technique are low sensitivity and resolution, which make it unable to provide detailed biological information. To offset the shortcoming and expand the bioimaging applications, attaching fluorescent probe (subcellular imaging) and MRI probe (noninvasive imaging) simultaneously to MSN is an important task. We adopt a strategy that simultaneously fuses the amorphous silica shell of Fe3O4@SiO2 nanoparticles with a fluorescein isothiocyanate (FITC) incorporated MSN. The utility of multifunctional Mag-Dye@MSN resides in their ability to combine organic/inorganic and diagnostic/therapeutic components within a nanoscaled size. Looking forward, we believe Mag-Dye@MSN could be a potential candidate for MRI contrast agent and targeted drug delivery. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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