Studies on the costituents of Chinese herbs-Gaultheria leucocarpa, Strobilanthes cusia, Ruellia tuberosa, Noni, Aster indicus, Fructus Psoraleae

Autor: Chwan-Fwu Lin, 林傳福
Rok vydání: 2007
Druh dokumentu: 學位論文 ; thesis
Popis: 95
The purpose of this study is to investigate the chemical constituents and quantitative analysis. There are six parts in this study. The first part is the study on the constituents of Gaultheria leucocarpa Bl. f. cumingiana ( Vidal ). The methanolic extract of the aerial part of G. leucocarpa (Ericaceae) was separated by column chromatography to afford seven compounds, including 12-hydroxy-13-acetyl-8,11,13-podocarpatrien -18b-oic acid (GL-1), 4b,12-hydroxy-13-acetyl-8,11,13-podocarpatriene (GL- 2), 5-methoxy-9b-xylopyranosyl-(+)-isolariciresinol (GL-3), schizandriside (GL-4), lyoniside (GL-5), vanillic acid (GL-6), scopoletin (GL-7). The structures of new compounds GL-1~GL-3 were established by spectral analyses. The known compounds GL-4~GL-7 were identified by comparison of their spectral data with those in the literature. The second part is the study on the constituents of Strobilanthes cusia (Nees) O. Kuntze. The methanolic extract of the whole herbs of S. cusia （Acanthaceae） was separated by column chromatography to afford ten compounds, including 2-hydroxyquinazolin-4(1H)-one (SC-1), 2,4(1H, 3H)-quinazolinedione (SC-2), 4-methoxy-2(1H)-quinazolineone (SC-3), 2-hydroxy-1,4-benzoxazin-3-one (SC-4), 2-benzoxazolinone (SC-5), indirubin (SC-6), tryptanthrin (SC-7), lupeol (SC-8), betulin (SC-9), 1-hydroxy -3-methyl-5,10anthraquinone (SC-10). The structure of new compound SC-1 was established by spectral analyses. The known compounds SC-2~SC-10 were identified by comparison of their spectral data with those in the literature. The third part is the study on the constituents of Ruellia tuberosa L.. The methanolic extract of the whole herbs of R. tuberosa （Acanthaceae） was separated by column chromatography to afford eight compounds, including cirsimaritin (RT-1), cirsimarin (RT-2), cirsiliol 4’-glucoside (RT-3), sorbifolin (RT-4), pedalitin (RT-5), Betulin (RT-6), Vanillic acid (RT-7), Indole-3- carboxaldehyde (RT-8). The structures of these compounds were identified by comparison of their spectral data with those in the literature. Furthermore, these compounds were also examined for their antiproliferative effects on HepG2 and KB cancer cell lines. Compounds cirsimaritin (RT-1) and cirsiliol 4’-glucoside (RT-3) showed cytotoxic effects against the KB cell line with IC50 values of 95.7 and 36.4 mM respectively, while cirsimarin (RT-2) exhibited a cytotoxic effect against against the HepG2 cell line with an IC50 value of 81.5 mM. The fourth part is the study on the constituents of Morinda citrifolia. The ethanolic extract of the fruit of Morinda citrifolia (Rubiaceae) was separated by column chromatography to afford fourteen compounds, including 1,6-dihydroxy-5-methoxy-2-methoxymethylanthraquinone (MC-1), 1,5,7- trihydroxy-6-methoxy-2-methoxymethylanthraquinone (MC-2), isoamericanoic acid A (MC-3), americanin A (MC-4), americanin D (MC-5), 3,3’- bisdemethylpinoresinol (MC-6), luteolin (MC-7), scopoletin (MC-8), 3-methoxy-2-hydroxyl-cinnamaldehyde (MC-9), p-cresol(MC-10), p-hydroxybenzoic acid (MC-11), p-hydroxybenzaldehyde (MC-12), 3-methoxy-4-hydroxy-benzaldehyde (MC-13), 2,5-dihydroxy-4-methoxy benzaldehyde (MC-14). The structures of new compounds MC-1~ MC-3 were established by spectral analyses. The known compounds MC-4~ MC-14 were identified by comparison of their spectral data with those in the literature. The fifth part is the study on the constituents of Aster indicus L.. A. indicus. The ethanolic extract of the whole herbs of A. indicus L.(Compositae) was separated by column chromatography to afford thirty-one compounds, including 4-hydroxy-3-[1-(methoxycarbonyl)vinyloxy]benzoic acid (AI-1), 5-(1-carboxylvinyloxy)-2-hydroxybenzoic acid (AI-2), 4-allyl-2,6- dimethoxyphenyl 3-methylbutanoate (AI-3), 4-Allyl-2-methoxyphenyl 2-methylbutanoate (AI-4), 3-(1-carbonylvinyloxy -4-hydroxybenzoic acid (AI-5), 4-hydroxybenzaldehyde (AI-6), vanillin (AI-7), vanillic acid (AI-8), friedelin (AI-9), trans-cosanyl ferulate (AI -10), methyl 3-O-caffeoyl quinate (AI-11), 1H-indole-3-carboxaldehyde (AI-12), methyl 3-O-feruloylquinate (AI-13), methyl trans-ferulate (AI -14), arillatoses B (AI -15), erulic acid (AI -16), p-coumaric acid (AI -17), 1H-indole-3-carboxylic acid (AI-18), kaempferol-7-O-b-D-glucopyranoside (AI-19), isoquercitrin (AI-20), apigenin-7-O-(6²-methyl ester)-glucuronide (AI-21), kaempferol (AI-22), quercetin (AI-23), 4-hydroxybenzoic acid (AI-24), 3,4-dihydroxybenzoic acid (AI-25), rutin (AI-26), nicotiflorin (AI-27), methyl 3,4-di-O-caffeoyl quinate (AI-28), 3,5-di-O-caffeoylquinic acid (AI-29), methyl 3,5-di-O-caffeoyl quinate (AI-30), 1,3-di-O-caffeoylquinic acid (AI-31). The structures of new compounds AI-1~AI-4 were established by spectral analyses. The known compounds AI-5~AI-31 were identified by comparison of their spectral data with those in the literature. The last part is the study on the bakuchiol, psoralen, and angelicin content of Fructus Psoraleae and its commercial concentrated products. Bakuchiol, psoralen and angelicin are bioactive constituents of Fructus Psoraleae (Buguzhi, the seeds of Psoralea corylifolia). The present study provides details of an HPLC method for the separation of bakuchiol, psoralen, and angelicin in Fructus Psoraleae. The relative standard deviation of the marker substances for intraday and interday analyses were 0.32~1.50 % and 0.20~1.03 %, respectively. The bakuchiol, psoralen and angelicin contents ranged between 36.29~71.06, 2.54~13.07 and 2.27~9.21 mg/g for ten raw material samples of Fructus Psoraleae, and 0.62~21.16, 0.63~5.23 and 0.67~5.37 mg/g for eight commercial concentrated products of Fructus Psoraleae.
Databáze: Networked Digital Library of Theses & Dissertations
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