Effect of auxins on peroxidase gene in hypocotyl cuttings of soybean during adventitious root formation
| Autor: | Chien-Hung Chou, 周建宏 |
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| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 The auxins promoted rooting in soybean (Glycine max L.) hypocotyls. Different auxins induced adventitious roots dissimilar development. Indole-3-butyric acid (IBA) treatment increases root number dramatically while simultaneously stimulating root formation along the whole soybean hypocotyl. Indole-3-acetic acid (IAA) and naphthaleneacetic acid (NAA) at the same concentration even reduce root number, and the roots formed most appear in the lowermost part of the cutting base. At the end of the treatment, on the day 2, the levels of IAA increased dramatically in the IBA-treated tissues. The content of IAA in the IBA-treated tissues is about 1.5-fold higher than that of the control tissues. The activity of anionic peroxidases (POX, pI 3.7) and cationic POX (pI 8.5) was significantly inhibited by exogenous auxins during the induction of adventitious roots on the day 2 and day 3. We focus on a cationic POX (pI 8.5) that was used PCR-based genome walking method to find its promoter sequence and analysis its cis-elements. The pI 8.5 POX gene promoter contains two sites that are homologous to sequences commonly found in auxin response elements. Once POX gene expression is suppressed by auxins before adventitious rooting, the endogenous IAA may increase, which would result in the increased level of endogenous IAA. The increased IAA and the exogenously applied auxins are working together to stimulate cell division and elongation in auxins-treated tissues during the induction of adventitious roots. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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