The physiological consequences of temperature adaptation and different phosphoglucose isomerase genotypes in mullet (Mugil cephalus)
| Autor: | Shao-Ju Weng, 翁紹儒 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Two types of mullet, the migratory (M-type) type with Pgi-A100 and resident (R-type) with Pgi-A135, live in different temperature environments around the coastal and estuaries of Taiwan. In this study, we experimented on mullet with 3 genotypes, Pgi-A 100/100, 100/135 and 135/135, and quantifying expressive differences of HSP70 to infer the physiological response. We can go a step further to identify two group of resident type and one migrant type using mtDNA 16s RNA-RFLP. After treating with low temperature for 4 hours, the 100/135 and 135/135 had higher level of HSP70 than 100/100 individuals(P< 0.05); after treating with high temperature for 4 hours, the HSP70 of 100/135 expressed slightly higher than 135/135 but no significantly different between them (P > 0.05). The individuals with Pgi-A135 allele were treated at stressful temperature condition for 48 hours. The HSP70 expression would exponentially increased during 0-8 hours after changing from 20°C to 35°C or 5°C. After 8 hours, HSP70 expression of Pgi-A135 decreased at 35°C but it still had great quantity of expression at 5°C. Therefore, Pgi-A alleles frequency variation among different types of mullet might be due to the force of natural selection. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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