Reducing the Specimen-induced Spherical Aberration by Polarized Photon-pairs Confocal Laser Scanning Microscope
| Autor: | Chi-hui Chang, 張積暉 |
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| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Refractive-index mismatch in conventional confocal microscopy produces severe degradation on axial resolution of sectioning image because the spherical aberration is induced by specimen. In this study, we propose a polarized photon-pairs confocal laser scanning microscope (PPCLSM) in which a two-frequency linear polarized photon-pairs (LPPPs) laser beam is produced. The common-path propagation of LPPPs integrated with optical heterodyne technique can reduce the spherical aberration. Therefore, the better axial resolutions of the sectioning image are produced. In the experiment, a verification and comparison between PPCLSM and conventional confocal laser scanning microscope (CCLSM) on the ability of reduction of spherical aberration induced by cover glass and by slide are demonstrated experimentally. Finally, the ability of PPCLSM which can decrease the spherical aberration based on the common-path propagation of LPPPs is discussed. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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