The study of Trichomonas vaginalis and human cervicalcancer cell line co-culture system in vitro
| Autor: | Yu Ching Hsuan, 余婧萱 |
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| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Trichomonas vaginalis, a protozoan parasite of the urogenital -vaginal tract, is the causative agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in human. In male, the trichomoniais is usually asymptomatic, although it may cause irritating urethritis or prostatitis. In female, trichominiasis is associated with a wide spectrum of clinical signs ranging from a relatively asymptomatic state to severe vaginitis with a foul-smelling vaginal discharge. T. vaginalis may act as a potential catalyst in the acquisition of secondary infection such as that caused by human papillomavirus, the organism responsible for the pathogenesis of cervical cancer. The adherent clump of this protozoan will destruct the epithelial cell and induce pathogenesis by contact-dependent cytotoxicty. A co-culture system of T. vaginalis and human cervical epithelium cancer cell line (Z172 cell) has been established in this study. Both of the protozoan and host cell grow well in the same culture condition and atmosphere. When Z172 cell exposure under T. vaginalis attack, the morphology of the host cells become round shape, shrinkage, detach, and part of the cells are died. The single Z172 cell is easier attacked by T. vaginalis then colonial cells. The more co-culture time and the more adhesion rate were observed in this study. After 12 hours’ co-culture, the adhesion between protozoan and cell become the most significantly than other time points observation. Does the pathological changes of the Z172 cell are derived by the physical or chemical after the adhesion of T. vaginalis are needed to more studies in the future. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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