The intra-specific variation for anatomy of stem and leafof Vitis thunbergii Sieb. & Zucc. in Taiwan
| Autor: | Wei-Rui Chen, 陳韋睿 |
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| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 53 V. thunbergii Sieb. & Zucc. collections, planted at same field were used to analyze tissue structure of leaf and stem, raphides and druses through paraffin sectioning, and then executed duster analysis and principle component analysis through software MVSP in order to understand the variation among collections and provide references of conservation, breeding, product development and utilization. The analysis results were summaries as below: 1. V. thunbergii has one layer upper epidermis、lower epidermis、palisade tissue and spongy tissue in leaf, particularly epidermis of midrib usually contains non- glandular hair. Upper epidermis is constructed by 6 to 10 layers parenchyma, whereas lower epidermis is 5 to 8 layers. Vascular bundle respectively and superlatively distribute as ring-shape, have adaxial vascular bundle at adaxial surface and 3 abaxial vascular bundle at positive side. 2. There usually have non-glandular hair on petiole epidermis, which has one groove on adaxial surface. Cortex contains 5 to 7 layers parenchyma. The couples of cortex vascular bundle distribute along adaxial surface groove of petiole, however, others distribute superlatively as ring-shape including 2 adaxial vascular bundle and 5 abaxial vascular bundle. 4 undeveloped or unidentified secondary abaxial vascular bundle distribute among primary abaxial vascular bundle. 3. There usually have non-glandular hair on epidermis of stem developed oval-shape and non-gibbous. Caliber of stem is perpendicular to pith. Cortex contains discontinuous fiber bundle. Xylem and phloem are separated by ray and are respectively bunched up. The number of pith ray is from 18 to 26. Phloem contains 0 to 3 fiber bundle. 4. All tissues of organ leaf, petiole and stem contain tannin cell, raphides and druses. Tannin cell distributes principally in cortex of petiole and stem, and phloem. Raphides distributes principally in cortex of petiole and stem. Druses distribute principall in phloem tissues of stem, and secondarily in cortex of stem and petiole, and vascular bundle. 5.The investigation item with maximum variation of 4 tissues are respectively tannin cell of leaf and midrib、 raphides in vascular bundle of petiole and raphides in xylem of stem. 6.The tannin cell number of cortex, vascular bundle and pith of midrib, petiole and stem respectively has very significant positive correlation. However there is no correlation among organs. 7.Raphides of cortex and pith and the druses in cortex, vascular bundle and phloem of petiole has very significant correlation. Druses of cortex and phloem of stem has very significant positive correlation, whereas xylem has significant negative correlation. The correlation analysis result among organs indicates that raphides in cortex of petiole and stem pith, and petiole pith and stem cortex and druses in cortex of petiole and midrib has significant positive correlation. 8. According to the cluster analysis result, 53 V. thunbergii collections are clustered into 4 groups. First group included 3 collections has more tannin cell in all tissues of petiole. Second group included 26 collections have more tannin cell in cortex of stem, less tannin cell in xylem of stem. Third group is collections VT-72, and has more tannin cell in cortex of stem, less tannin cell in vascular bundle and pith of petiole. Fourth group included 23 collections have more tannin cell in xylem of stem. 9.The result of PCA indicated that first principal component represents raphides in pith of petiole, druses in cortex of petiole and raphides and druses in cortex of stem, and the second principal component analysis represents tannin cell in cortex and vascular bundle of petiole, tannin cell in phloem of stem and druses in cortex of stem. Furthermore, 53 collections are clustered into 3 groups except 2 collections according to 2D plot analysis between first second principal component analysis. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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