Gene Methylation Profiling of Lung Cancer Cells using CpG Islands Microarray Analysis
| Autor: | Kai-Tse Chiu, 邱鎧澤 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 The process of DNA methylation is a commom existed mechanism in life to modulate epigenetic express. Different degrees of methylation in chromosomes were identified. The phenomenon is explained to modulate the different physiological express by diversity methylation degrees in different kind of species. And each of them has their own dependent physiological express. However, the genetic modultated mechanisms of the methylation are not clearly understood. This study is to analysis the GpG sites of 56 paired non-small lung cancer specimens provided by Taichung Veteran Hospital, and to discover the interaction between the telomerase activation and DNA methylation, and thus, to discover the methylation process in these specimens. We first analysis the methylation situation in telomerase-related gene, like the promoter of hTERT, hTRF1 and hTRF2. The result showed significant relationship between the methylation situation and the activity of the telomerase, and also the tumor stage. When hTERT was meththylated, the telomerase activation was supressed(p=0.038). We also noted that during the process of carcinogenesis, the cells would hypermethylated first (p |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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