Evaluation of Japanese encephalitis virus NS3 protein to induce apoptosis (HeLa and Vero) and transformation (NIH 3T3).
| Autor: | Yen-Hsieh Chen, 陳彥劦 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Previously reports indicated that flaviviral non-structural protein 3 (NS3) displays serine protease, triphosphatase and RNA helicase activities and serveral flaviviral NS3 had further found their ability to triggercaspase-dependent apoptosis. To clarify if Japanese encephalitis virus (JEV) NS3 and its helicase domain alone can induce apoptosis, we applied mophology observation, dye exclusion and caspase activity methods. After liposome-based transfection, JEV NS3-expressing cells exhibit the mophology of apoptotic cells, i.e. cell shrinkage, chromatin condensation, and nuclear fragmentation. Moreover, these cells also show their in situ caspase-9 and downstream caspase-3 activities. Additionally, former research had reported that NS3 protein of Hepatitis C virus (HCV) could induce transformation of NIH 3T3 cells. Also, those JEV NS3-expressing 3T3 cells loss contact-inhibition, become ancharage-independent, and shape into cell colonies in soft-agar, which all correspond with the mophology of transformed cells. In conclusion, JEV NS3 protein and its helicase domain are capable of triggering caspase-9-based apoptosis in HeLa and Vero cells and transformation in NIH 3T3 cells. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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