The effect of alcohol on the differentiation of neural stem cell.
| Autor: | Pei-Jun Xie, 謝佩君 |
|---|---|
| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Neural stem cells (NSCs) defined as immature cells with self–renewing and multipotent abilities. In the mammalian brain, most neurons are generated by NSCs in the later embryonic period. Recent studies have described the possible relevance of impaired NSCs’ functions to the pathophysiology of psychiatric disorders, including alcoholism. Therefore, the aim of the present study was to investigate the effects and mechanism of ethanol on the differentiation of NSCs. NSCs obtained from 14–day–old rat embryos were propagated as neurospheres and cultured under differential conditions with or without dbcAMP and various concentrations of ethanol（50–150 mM）. Ethanol exposure slightingly increased NSCs death at 150 mM, while 50 to 100 mM ethanol reduced neuronal differentiation without affecting the viability of NSCs. Western blot analysis revealed that ethanol suppressed phosphorylation of extracellular signal–regulated kinase（ERK）and Tuj–1 expression. Furthermore, morphological observation demonstrated that ethanol reduced neurite outgrowth. Previous studies showed that dbcAMP–induced neural differentiation required ERK signaling. Based on these results, we postulate that ethanol disrupts neural differentiation via ERK–dependent pathway. It is possible that ethanol exposure impaired neural network by reducing neurite outgrowth, which may contribute to an explanation of the defects in brain function often observed in fetal alcohol syndrome. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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