Study of a Porous Bi-layered Chitosan Membrane Contained Collagen I Nano-spheres for Skin Tissue Engineering
| Autor: | Wei-Ju Liao, 廖偉茹 |
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| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 The purpose of this study was to prepare and evaluate a novel degradable membrane with porous bi-layered structure seeded with fibroblast for skin tissue engineering. In order to improve the biocompatibility of chitosan membranes, a low-toxic cross-linking agent, genipin was used in the present study. For obtaining a porous bi-layered structure, the genipin crosslinked chitosan membrane (CG membrane) was then immersed into the liquid nitrogen. For analyzing the properties of CG membrane and determining the optimal concentration of genipin solution used in the CG membrane, the swelling ratio, fixation index, in-vitro degradation rate, and in-vitro culture of the CG membrane with fibroblast were explored in this work. Results indicated that CG membrane treated with A wt % GP had superior porous bi-layered structure, adequate swelling ratio, porosity, pore size, and better biocompatibility. Further, collagen nano-spheres have been coated on porous bi-layered CG membrane to produce the CGC membrane. In vivo histological assessment results revealed that epithelialization and remodeling of the wound were achieved by covering the wound with the porous bi-layered CGC membrane, indicating that it could probably be a potential skin substitute for skin tissue engineering. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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