Bioeffect mechanism of Baicalein on Angiogenesis of Endothelial Cells
| Autor: | Yun-Chih Hsieh, 謝昀志 |
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| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 Baicalein, a flavonoid present in the root of Scutellaria baicalensis GEORGI, has been reported to inhibit proliferation in rat heart endothelial cells. In this study, the effect of baicalein on cell angiogenesis was examined in vivo and in vitro experiment to observe the bioeffect mechanism in endothelial cells. We found baicalein inhibited angiogenesis well but pretreatment with baicalein significantly induced the formation of tube-like structures both in vitro and in vivo. The activation of invasion was increased by pretreatment with baicalein that change endothelial cells and ECM interaction. Baicalein-mediated MMPs activity and decreased TIMPs protein levels and mRNA levels by promote of gelatinolytic activity. Thus, our observations demonstrated that baicalein drastically inhibited the migration and enhanced the adhesion of rat heart endothelial cells, possibly by up-regulation of the integrin α5β1, integrin αvβ3 and vinculin, and promotion of actin reorganization and focal adhesion contact formation. We have provided a evidence to indicate baicalein control angiogenesis by up-regulation of integrins on the surface of endothelial cells. Baicalein is an angiogenesis promoter and potentiator in vivo, may use as an angiostatic and angiogenic. Furthermore, we constructed ABLIM1, TMOD1, CYR61, ENO1, MBP1, PLSCR1, SLR417, and RAI3 expression and Yeast-Two hybrid system plasmids. We also constructed MAP2K1, MAP2K2, MAPK1, MAPK3, MAPK8, MAPK9, MAPK14, RARα, RARβ, RARγ, RXRα, RXRβ, RXRγ, ABLIM1, ENO1, TMOD1, RAI3, and Fos siRNA plasmids in plasmid U6 and pSIREN system witch promoted by U6 promoter. We proposed to observation specific gene overexpression and knocksown in cell. Using these plasmids to regulate medical molecules involved in protein-protein interaction between cells and tissue expression. Thus, we explain to find new therapeutically mechanisms in vascular disorder and cancer angiogenesis. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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