AN INTERSTITIAL FLUID APPROACH TO DISCOVER BIOMARKER CANDIDATES FOR HUMAN HEPATOCELLULAR CARCINOMA
| Autor: | Chen chia hua, 陳佳華 |
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| Rok vydání: | 2007 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 95 One of the main missions of clinical onco-proteomics is not only to discover more biomarkers for early stage diagnosis of disease but also to provide potential targets for drug discovery and therapeutic intervention. According to epidemiology studies, HCC has been the major leading cause of cancer death in the world. It has been widely known that HCC is strongly correlated to HBV/HCV infection and other causalities, however, the molecular mechanisms of hepatocarcinogenesis are still unclear. High sensitivity and specificity HCC biomakers are useful in HCC early detection and hopefully can increase patients’ survival rate. The markers, such as a-fetoprotein, used so far have limited utility when detecting early stage HCC. Therefore, finding novel markers with higher sensitivity and specificity is urgently needed. We collected the tumor (TL) and nontumor (NL) tissue lysate and also the interstitial fluid perfusing the HCC microenvironment from the surgical resects of the tumor (TIF) and para-tumor liver tissues (NIF) in nine cases of hepatoma, and analyzed them using 2-DE and MALDI- TOF MS. The amount of five proteins- PRDX1, CyPA, ERp57, RBP4 and 14-3-3 sigma were frequently found to be higher level in TIF as compared to those in the NIF. The results were further verified via immunoblotting analysis. HCC and cirrhosis patients’ serum have higher cyclophilin A level than normal ones. Our findings suggest that these five proteins, particularly cyclophiln A, could be potential markers for diagnosis of HCC. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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