Surface Modification of Bio-materials by Cold Plasma Treatment and UV Induced Graft Polymerization
| Autor: | Su-Chen Chen, 陳素真 |
|---|---|
| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 In this study, we used plasma treatment and photo-induced grafting polymerization technologies to surface modify the character of materials for applications to biosensor and biomaterial fields. For biomaterial application, we fabricate an easy-strip gelatin scaffold on surface of PNIPAAm gel /polypropylene (PP) nonwoven to application in wound dressing. Nonwoven have wide industrial application due to its cheapness, low density, excellent mechanical properties and high porosity. However, due to lack of chemical functionalities, the hydrophobic natures of these surfaces have restricted the use of polypropylene. Hence, in this study, we used thermal-sensitive culture scaffolds to develop a novel easily-stripped cell membrane manipulation to cure different cells. The cell membrane was fabricated by Ar plasma treatment and photo-induced graft-polymerization thermo-sensitive monomer N-isopropylacrylamide (NIPAAm) to modify the surface character of PP nonwoven. Then, after immobilization of the porous gelatin as cell membrane, it can be easily removed from PNIPAAm gel when it is immersed in cold water. The stability of the carrier materials under in-vitro conditions and proliferation of the cells was investigated by SEM and MTT test. Microscopic investigation showed that corneal epithelium cell morphology is stretched out along the length of the gelatin and even penetrated into the inner gelatin organization. Furthermore, the results of SEM and HE staining also showed that corneal epithelium and fibroblasts cells could attach to the scaffold. The porous structure of the gelatin scaffold provided an excellent space that was suitable for cell growth in this circumstance. Thus membrane-transferred corneal epithelium stem cells can be used to regenerate corneal epithelium in the eye or fibroblasts cell in the skin. Secondly, biosensor technology offers the possibility of monitoring hybridization in real time and with high selectivity. In this study, we developed a sensing system for detection of Vibrio parahaemolyticus using its oligonucleotide probe immobilization on the gold electrode surfaces of QCM. However, since the QCM surface was an inorganic substance, it is difficult to immobilize the oligonucleotide probe. In this study, the plasma surface modification of QCM for deposition of hexamethyldisilazane (HMDSZ) films as an interlayer was investigated. This provided good adhesion to the substrate and had a uniform structure. Then, we use chemical bonding to immobilize the DNA probe on the amino surface via a glutaraldehyde (GA) linker. The effect of post-treatment of the immobilized process, such as surface graft AAm and PEI treatment, is compared and the determinate limit for this way is discussed. The surface properties after each modification process were verified by water contact angle and ESCA spectra. The results demonstrate that the shift of resonance frequency of QCM was improved via subsequent graft polymerization of AAm and PEI treatment onto the electrodes. The QCM sensor after plasma deposition and surface modification could provide detection sensitivity up to 86 ng/ml and retained 90% detection sensitivity after 11 days of storage at 0oC. After washing with 0.1 M NaOH solution and 7 of repeated uses for detection, the regeneration rate of QCM could be as high as 60%. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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