Inhibition of Melanogenesis in B16 cells by SACCHACHITIN, a Cell Wall Component from Ganoderma tsugae
| Autor: | Hsiang-ning Chang, 張湘寧 |
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| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 SACCHACHITIN was prepared from the waste residue of the fruiting body of Ganoderma taugae, was used in our previous study to inhibit matrix metalloproteinases and lipase. In the present study, SACCHACHITIN was estimated for its effects on the tyrosinase activity and expression. In the mushroom tyrosinase inhibition assay, the results indicated that 2.5 mg/ml of ascorbic acid had strong inhibition of mushroom tyrosinase. In different source of chitin, only SACCHACHITIN showed a potent tyrosinase activity inhibition, IC50 value was 17.2 mg/ml. In the cell culture assay, SACCHACHITIN showed neither morphological alteration nor disorder in proliferation. These evidences elucidated that SACCHACHITIN was not cytotoxic and safe toward the growth of B16 mouse melanoma cells. Use 25nM -MSH induce melanogenesis in B-16 cells could elevate 3.37 times of melanin content. The results of inhibitory effect of SACCHACHITIN with no inducer and with inducer -MSH on melanin biosynthesis and tyrosinase activity were observed in B-16 mouse melanoma cells all in a dose-dependent response reaction. Western blot was employed to ensure SACCHACHITIN inhibited B16 cells tyrosinase protein expression, and the results indicated that 2.0 mg/ml SACCHACHITIN had obviously inhibitary effect. In the same experiment, SACCHACHITIN in small particle ( average size: 10 μm ) seemed in clined to enhance the inhibition of tyrosinase protein expression. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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