Determination of tannic acid based on on-line optical detection system
| Autor: | Chien-Yu Chung, 鍾建育 |
|---|---|
| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 Two on-line systems have been setup in this research for determination of tannic acid content in green tea infusion and biological activation of protein and tannic acid aggregation in tea. In fluorescence quenching method with flow-injection system which has been applied for detection of real samples within a linear concentration range of 0.0-1.0 mg/ml and a R2 of 0.9928. The method is immune from the interference of the coexisting ascorbate in green tea infusion and temperature changing, and the analytical results were verified by Japanese official analytical method, ferrous tartrate method. In light scattering method with flow-injection system, experiment error such as drift of baseline and lacking of reproducibility can be solved effectively by cleaning procedure with SDS reagent for the effective removal of residual aggregation particles by tannic acid and BSA. Therefore, an on-line detection system with real time detection and cleaning ability have been setup successfully. The range of detection is 20-300 μg/ml, and astringent activation in tea can be known by aggregation of tannic acid and BSA |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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