Using proteomic techniques to investigate the action mechanisms of botanic hormone on osteoblasts
| Autor: | Ning-Jo Kao, 高寧若 |
|---|---|
| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 Osteoporosis, characterized by the loss of bone mass and strength leading to fragility fractures, has probably existed throughout human history but only recently became a major clinical problem as human lifespan increased. Osteoporosis can be divided into two types: One is common by estrogen deficiency of menopausal women whereas another type is found in people with severe calcium absorption insufficiency by increases with the age. Multiple pathological mechanisms have been demonstrated to induce bone density reduction causing the degradation of skeleton. Theses factors have increased risks of act and the incidence of patient's fragility fracture of osteoporosis. Studies have shown that “Hormone replacement therapy” application on menopausal women with osteoporosis may enhance the risk of developing breast, endometrial and ovarian cancers. Therefore, alternatives to hormonal therapies with similar effect of estrogen were developed such as phytoestrogen having similar chemical structure to mammalian estrogen to prevent the complications induced by estrogen. However, the action mechanisms of these phytoestrogens are not fully clear. Therefore, in this study we will attempt to explore and compare the action mechanisms of estrogen and phytoestrogens on osteoblast-like cells, MG63, by using proteomic approaches including two-dimensional gel electrophoresis combined with mass spectrometry identification and protein microarray techniques to identified different expression of intra cellar proteins and secreted cytokines induced by estrogen or phytoestroens on MG-63 cell line, respectively. Our results showed that: intracellular proteins within the MG63 cells did reveal the difference among the testing compounds. However, the insufficient amount of protein causes the difficulty for identification by mass spectrometry. Another important finding is that MG63 cells secreted different cytokins and growth factors treated with different compounds, demonstrating the autocrine and/or paracrine functions of these soluble mediators may play important roles during osteogenesis. Further studies need to be performed to elucidate their roles on preventing osteoporosis in vivo. Key words : MG63 osteoblast-like cellsestrogen、phytoestrogen 、proteomics、 |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|