Evaluation of Biosurfactant Efficiency in Removal of Nosocomial Microbes in Biofilm
| Autor: | Chi-Wei Lee, 李紀緯 |
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| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 Taiwan’s CDC (Center for Disease Control and Prevention) has reported that nosocomial infection rate is around 4 % when hospitalized for one day, and up to 15% in the intensive care units while the risk resulting from the contact with biofilm is considered most noteworthy. Although various measures have been adopted to sterilize and disinfect microbes in different pathways, they have not been considered complete and free of concerns. This study was aimed to evaluate the biosurfactant, a redeveloped biological substance, in the inhibition of the microbial growth and to establish a simple system for controlling and monitoring microbes attributable to nosocomial infection. Two clinical strains used in the study, Pseudomonas aeruginosa 91P8 and Staphylococcus aureus 920, were cultivated as biofilm for testing. Rhamnolipid, a biosurfactant, was added into biofilm to evaluate the inhibition efficiency under different concentrations and for different durations. Other disinfectants commonly used in hospital including povidone iodine, alcohol, and hypochlorite, were also used to examine the disinfection capability with co-existence of biosurfactants. In inhibition assay, P. aeruginosa and S. aureus biofilm were reduced by 69.9% and 57.0% of biofilm growth respectively when using 16 μg/ml Rhamnolipid. Use of iodine and biosurfactant mixture reduced the survival percentage of biofilm to zero, in comparison to use only iodine as a single disinfectant. Application of alcohol and hypochlorite decreased the biofilm survival percentage by 7% and 10%, respectively. Our study showed that using the biosurfactant could effectively increase disinfection capability, and therefore reduce the risk of nosocomial infection caused by biofilms in hospitals. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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