Carbohydrates Determination by capillary electrophoresis with electrochemical detection
| Autor: | Ching-Hsun Chuang, 莊清訓 |
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| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 In this study, we developed a capillary electrophoresis(CE) separation combined with electrochemical detection(ECD) method for the determination of mannitols in Cordyceps militaris. A simple, fast, reliable and reproducible method based on capillary electrophoresis with electrochemical detection(CE-ECD), for the determination of carbohydrates in Chinese traditional drugs was described in this work. A copper microelectrode was used as working electrode. The effects of the potential of the working electrode, and concentration of running buffer, electrolytes composition of running buffer, separation voltage, and injection time on CE-ECD were investigated. In this method, both the separation and the detection required the use of alkaline electrolyte with 20cm long, 20μm i.d. fused silica capillary. With the optimized condition of separation and detection, the migration time of mannitol is 315(±7)s determination with dynamic range of 10.00mM to 0.10mM and of 0.05mM(LOD)limit of detection. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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