The relationship among passive maternal smoking, newborn DNA damage and birth outcome
| Autor: | Huai-Chih Tsui, 崔懷芝 |
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| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 94 Background: Previous studies have shown that cigarette smoking contributes to various harmful effects for pregnancy maternity and neonates, and it remains a major public health problem. In this study, we examined the relationship among maternal environmental tobacco smoking, newborn DNA damage and the associated birth outcomes. Methods: This study comprised 383 paired samples of pregnancy maternity and newborns. For the three trimesters, blood and urine specimens were collected and structured questionnaire were administrated. Further, neonatal serum samples and their birth outcomes (including height, weight, head circumference etc.) were collected as well with consent. Genotype analyses of CYP2A6, CYP1A1, GSTT1, GSTM1, and NAT2 were performed by PCR-RFLP. Finally, we utilized the comet assay to measure the degree of newborn DNA damage. Results: Among the 383 participants, 175(45.7%) were non-smokers, 184(48.0%) exposed to ETS and 24(6.3%) were smokers. The ETS-exposed women were adversely associated with neonates in various birth outcomes, but not significant. Infants who were born to ETS-exposed women had smaller head circumference than those to women who were non-smokers. The degree of newborn DNA damage was not related to metabolic polymorphic genes. Analytical findings indicated that the levels of newborn DNA damage in smoking and ETS-exposed groups were higher than that in non-smoking group. The difference was particularly significant for the ETS vs. non-smoking groups (69.7±42.3 vs. 54.0±33.8, p=0.004). The increase in DNA damage levels was positively associated with an increased in infant adverse birth outcome. ETS-exposed women, in particular for those with serious DNA damage, had significantly lowered birth in weight (-262.2g, p=0.002), height (-1.3cm, p=0.006), and head circumference (-0.9cm, p=0.007). Conclusion: Our results suggest that infant birth outcomes are adversely correlated with maternal ETS-exposure. In addition, newborn DNA damage is significantly associated with maternal ETS-exposure. On the other hand, relationships among newborn metabolic polymorphic genes, newborn DNA damage and birth outcomes are not explicitly observed. |
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