Immunoregulation of 26KDa GST of Schistosoma japonicum (Chinese strain) on dendritic cells and macrophages
| Autor: | Hsiu-Hsia Lee, 李秀霞 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 Glutathione S-transferase (GST) is generally recognized by WHO as one of the major candidate vaccine antigens against Schistosome infections. The dendritic cells (DCs) and macrophages (Mψ) are antigen -presenting cells (APC), especially dendritic cells, acquire antigens in peripheral tissues and migrate to lymphoid organs where they present processed peptides to T cells, and induce innate and then acquired immune responses. In order to understand the interaction and immuno -modulation of dendritic cells, macrophages and 26KDa rGST vaccine antigen of Schistosoma japonicum, Flow cytometry and confocal laser scanning microscope were used to perform this study. The results showed that DC from mice immunized with 26KDa rGST antigen expressed strongly class II MHC and the CD8α+, but lesser on macrophages. The presentation of location of GST on DC is overlaped with class II MHC based on the obserotion of confocal laser scanning microscope. This result demonstrates that DC stimulated with rGST could process via incorporation of class II MHC molecule and present as GST antigen. Furthermore, we found that DCs of mice immunized with GST were re-stimulated rapidly and activated apparently while eggs produced, after seven weeks of infection. the CD8α+ marker started expressed on DCs continuously to chronic stage. In addition, the class II MHC increased markedly on macrophage after five and seven weeks of infection. These results suggested the antigen presenting ability of DC induced by rGST vaccine antigen come mainly from the GST of eggs, which lead to effective increase of host immune response against schistosome infection. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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