Pharmacokinetics of curcumin and its herbal analysis

Autor: Kuo-Yi Yang, 楊國義
Rok vydání: 2005
Druh dokumentu: 學位論文 ; thesis
Popis: 93
The aim of the study was to investigate the pharmacokinetics of curcumin in awake and freely moving rats and its herbal analysis. The method based on an automated blood sampling system (ABS system) coupled to a validated liquid chromatography with tandem mass spectrometry (LC-MS/MS) electrospray ionization (ESI) method to monitor curcumin in rat plasma. The yields of the curcumin and demethoxycurcumin from commercial curcumin crude extract were 37% and 4%, respectively, which were identified by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and LC-tandem mass spectrometry. We also developed a validated liquid chromatography with tandem mass spectrometry (LC-MS/MS) electrospray ionization (ESI) method to measure curcumin and demethoxycurcumin from herbal extracts of Curcumin longa L. The results indicate that the contents of curcumin in Curcuma longa L. extracted by water, 50 % ethanol and 95 % ethanol were 0.11 ± 0.03, 14.4 ± 0.9 and 165.3 ± 2.1 mg/kg, respectively. The contents of demethoxycurcumin in Curcuma longa L. extracted by water, 50% ethanol and 95% ethanol were 0.13 ± 0.01, 3.9 ± 0.2 and 43.7 ± 0.3 mg/kg, respectively. In pharmacokinetic study, the analytes were separated on Agilent C18 column (150 × 4.6 mm I.D., 5 μm) analytical column and eluted with acetonitrile-10mM HCOOH (70:30, v/v). The limit of quantification of curcumin in rat plasma was determined to 5 ng/mL and the linear range was 5-10000 ng/mL with a coefficient of correlation > 0.997. In addition, the area under the concentration versus time curves were 7.2 ± 1.2 min �慊/mL and 3.6 ± 0.6 min �慊/mL for curcumin intravenous doses of 10 mg/kg and oral dose of 500 mg/kg, respectively. The oral bioavailability of curcumin in freely moving rat was 1%. About 41% of curcumin oral dose was eliminated through feces and the urinary excretion of curcumin was almost undetectable.
Databáze: Networked Digital Library of Theses & Dissertations
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