The effects of phytoestrogen on osteoblast activity
| Autor: | Ya-Ying LI, 李雅嫈 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 Postmenopausal osteoporosis is a metabolic bone disease characterized by a decrease of bone mass after menopause, which may lead to spontaneous and traumatic fractures in still-active women. In postmenopausal women, the bone loss is sufficient to cause osteoporosis. Several studies have shown that estrogen replacement therapy (ERT) contributes to maintenance of skeletal mass and can reduce the risk of fractures in postmenopausal women. But long-term ERT has been associated with increased risk of cancer in estrogen target tissues, including the mammary gland and uterus. In recent years, find some composition in plant that similar to estrogen structure or the biological function. The research wants to probe into the estrogen in plant (phytoestrogen) and can replace estrogen and become the safer medicine in the treatment of the osteoporosis. In this study, we assessed the effects of the phytoestrogen on cell proliferation, ALP activity and calcium accumulation in osteoblast from neonatal mice. Furthermore, the cells transcriptionally expressed the detectable levels of collagen type I, ALP, OPG, OPGL, estrogen receptors (ERα and ERβ) mRNAs. The results presented herein characterize the cellular effects of phytoestrogen on bone tissue. Phytoestrogen induced a increase in osteoblast proliferation, with the optimum effects at 10-9 M. We tested osteoblasts with phytoestrogen and assessed the cellular expression of osteoblast-specific genes by real-time PCR. Phytoestrogen can decrease the bone mass loss by increase bone formation, which prevents the development of osteoporosis. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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