Studies on sex expression-related proteins in bitter gourd (Momordica charantia)
| Autor: | Ju-Chen Chia, 賈儒珍 |
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| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 Sex expression of bitter gourd (Momordica charantia L.), a monoecious species in Cucurbitaceae, is altered by exogenous application of plant growth regulators, with unkown mechanism of sex determination. In this study, we use 2-dimensional protein gel electrophoresis as the analysis method of sex expression-related protein in bitter gourd. To conclude different development stages in male and female flowers for analysis of protein expression, the morphology, length and microscopic structures of flower buds in M. charantia cv. Moon Shine were observed. Roots, stem, leaves, male and female flowers were used as materials for 2-dimensional electrophoresis. Among these, 8 flower organ-related proteins, 15 male flower-related proteins and 12 female flower-related proteins were obtained and numbered. In different development stages of male flowers, 51 development-distinctive proteins were found; 13 of these are male flower-related and 4 are female flower-related proteins. During female flower development, 31 development-distinctive proteins were found; 5 of these are female flower-related proteins, and 1 are male expression-related protein. On the other hand, shoot apex of bitter gourd at the 8-leaf stage treated with 0.54 mM naphthalene acetic acid (NAA) and 0.12 mM gibberellic acid (GA3) were used to analysis for protein expression. NAA inhibited some of the flower organ-related proteins and female flower-related proteins, and GA could induce them. Based on the molecular weight, pI value and expression level of the proteins, four expression type of sex expression-related proteins were defined. Group 1 proteins expressed constitutively during flower development. Protein amount of group 2 changed during male or female flower development. Group 3 proteins elevated during male and female flower development. Group 4 proteins elevated during female flower development and declined during male flower development. Group 1~3 proteins were possibly involved in male or female flower development, but less related with sex-differentiation. Group 4 proteins were found highly related to sex-determination due to their expression characteristics. The results showed that spot 18, 70 and 74 had close relationship with sex-determination and could be used for cloning of sex expression-related genes. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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