Study on the antibody production and detection to the major outer membrane protein of Vibrio anguillarum in grouper
| Autor: | Shui-Tsung Chen, 陳水聰 |
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| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 In Taiwan, the disease rate of farming fish has increased tremendously due to the way of high-density farming fishery, which resulted in high death rate of fishes and shells. The economic consequence has been devastating. Since vibrosis is the most commonly-encountered infectious diseases in fish, research related to vibrosis of farming fish has been intensively carried out worldwide. Researches include diseases caused by vibrio bacteria, pathogen recognition and detection, and vaccine development. Vibrio anguillarum is a Gram-negative bacterium which is classified into 10 serotypes based on its liposaccharides O antigens. In addition to the O-antigen, the major outer membrane protein (MOMP) of V. anguillarum has also been identified as a major antigenic determinant. Among these serotypes, O1, O2, and O3 serotypes are the most commonly found. We believed that the MOMPs of V. anguillarum may act as the targets for diagnosis and vaccine development. Their molecular masses are 41, 35, and 37 kDa for O1, O2 and O3 MOMPs, respectively. In this work the MOMPs of O1, O2 and O3 serotypes of V. anguillarum were individually purified by polyacrylamide gel electrophoresis (PAGE). Each has been used as antigen to elicit antibody from rabbit. The cross reaction of produced antibodies with those three antigens were checked by Western blot and immunochemistry assays. There is cross reaction between O2 and O3, but O1 has no cross reaction with O2 and O3. These results are consistent with the previous known serotypical properties among the three serotypes. Antibodies were found in grouper injected with either formalin-treated (inactivated) or live V. anguillarum and they reacted with the corresponding MOMPs of O1, O2 and O3 serotypes. Our results suggest that the MOMPs might be used as the diagnostic reagent for early detection of V. anguillarum infection in fish. They may be further tested for vaccine development in the future. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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