The alternations of chloride channel, ion transporter and gap junction under hyperosmotic stress in the astrocyte and brain of tilapia (Oreochromis mossambicus)
| Autor: | Shang-Lin Chang, 張尚麟 |
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| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 When Oreochromis mossambicus (tilapia) goes from a hypoosmotic (freshwater; FW) to a hyperosmotic (seawater, SW) environment, the osmolarity and the ionic concentrations of sodium and chloride ion in the Cerebral Spinal Fluid are raised. However, the regulating mechanism in fish brain responding to salinity changes remains unclear. This study attempted to test whether chloride channel, ion transporter and gap junctions in the astrocytes of tilapia brain were activated to alter the physiological response during hyperosmotic stress. Using semi-quantitative RT-PCR, the mRNA expressions of two chloride channels (ClC3 and ClC5), ion transporters (NKA?, NKCC1-?and NKCC1-β) and gap junctions (Cx43 and Cx35) in tilapia brain and astrocyte were investigated. After 25 ppt SW transfer, ClC3 expression in tilapia brain was significantly increased within 1 h (P |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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