Passivation Effects of Initial Fibroblast Adhesion to Titanium Alloy with Nano Roughness
| Autor: | Shang-Chieh Lu, 盧尚頡 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 This study aims at investigating the initial adhesive force between fibroblasts and passivated Ti6Al4V alloy of nano-surface roughness. Effects of four passivation treatments were examined: non-treated (HC), heated in 400°C air (HT), aged in 100°C de-ionized water (HA), and immersed in volume 34% nitric acid (HP). The chemical property of surface oxide is measured by X-ray Photoelectron Spectroscopy (XPS); the nano-surface roughness and morphology is measured by Scanning Probe Microscope (SPM); the surface wettability is measured by static contact angle assessment method; the initial adhesive force is measured by cytodetachment method; images of cell morphology is taken by Multi Scanning Electron Microscopy (M-SEM). The surface wettability is related to the content of basic Ti-OH group on the nano-surface of Ti6Al4V alloy. It has been observed that the higher the contact angle, the stronger the cell adhesion. By using SPM, it has been observed that different passivation treatments would modify the surface roughness of Ti6Al4V alloy differently, from 1.9nm to 7.4nm. By applying cytodetachment method, the initial cell adhesive force of fibroblasts was measured to be within 54 to 103nN for four different passivation treatments. Experimental results showed that different passivation treatment yielded different contents of basic Ti-OH group, wettability, and surface roughness, and they differently influenced the initial cell adhesive force and morphology of fibroblast on specimens of nano roughness. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|