Exposure of pentachlorophenol and its metabolite, tetrachlorohydroquinone leads to malfunction of splenocytes in mice
| Autor: | Hsiu-Min Chen, 陳綉敏 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 Pentachlorophenol (PCP) is a general biocide used extensively as a wood preservative. The major metabolite of PCP is tetrachlorohydroquinone (TCHQ). Dermal absorption is an important route PCP in human. PCP has been evaluated for effects on immune function in human, mice and rats. In human, malignant lymphoma and leukemia have been associated with occupational exposure. Lymphoma was induced in mice by dermal exposure of PCP. However, the precise mechanism is still unclear. Thereby, this study was designed to investigate the effects of PCP and TCHQ on immune system, particularly spleen in mice by dermal exposure. The result indicated that PCP and TCHQ affect splenpcytes and its immune function both in vivo and in vitro. In vivo, mice treated with PCP for 6 wks had increased spleen weight, S phase of splenpcytes and mitogen-induced cell proliferation. Mice treated with PCP for 12 wks decrease spleen weight, increased apoptotic splenocytes and inhibited LPS-induced proliferation. Spontaneous apoptosis was found in normal primary culture of splenocytes. We found a significant inhibition of apoptosis in culture splenocytes treated with TCHQ. Whereas, pretreatment of TCHQ inhibited splenocytes proliferation and secreted cytokines, including IL-2 and TNF-αstimulated by mitogen. Uncontrolled cell proliferation and inhibition of apoptosis in cells could be associated with the process of carcinogenesis. We suggest that malfunction of splenocytes induced by PCP and TCHQ could be one of the possible mechanisms of PCP-induced lymphoma in mice. Nevertheless, the detail mechanisms need to be further investigated. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|