Tissue distribution study of interleukin-10 family
| Autor: | Yi-Lin lee, 李宜霖 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 IL-10 family includes IL-10, IL-19, IL-20, IL-22, MDA-7 (IL-24), and AK155 (IL-26). These cytokines share not only structural homology but also receptor subunits and perhaps activities. Receptors for these cytokines belong to the class II cytokine receptor family. All these cytokines require two distinct receptor subunits for signaling. One puzzle surrounding the redundancy in receptor utilization by IL-24, IL-19 and IL-20 is that whether these cytokines induce identical signals downstream, or compete the same receptors for a different biological end-point. Much of this could depend on where and when the cytokines and their receptors are expressed. Thus, it is necessary to analyze the expression patterns of these cytokines and its receptors. Cytokines are involved in various inflammatory diseases such as psoriasis, asthma, atherosclerosis, rheumatoid arthritis (RA) and osteoarthritis (OA), in addition to the critical roles playing in tumor development. To evaluate the association of the expression patterns of these cytokine and its receptors with diseases, we performed immunohistochemical (IHC) staining to analyze protein level of IL-10 family and its receptors in clinical specimen. According the results of tissue microarray, we found that the expression of IL-20 is cell-specific in various tissues. And the expression pattern of IL-20R1 is almost the same as the expression pattern of IL-20. Besides, we also found that IL-20 is expressed in pancreatic acinar cells, which is involved in exocrine system. According to previous study, it is reported that acinar cells of the pancreas are the target of IL-22, another member of IL-10 family, and the receptor subunit of IL-22, IL-22R, is also expressed in the pancreas. The IL-22R is also a subunit of the functional receptor of IL-20, paired with IL-20R2, thus we want to evaluate if IL-20 plays any role in the pancreas. We perform RT-PCR to detect the expression level of the transcripts of IL-20 and its functional receptors in several pancreatic cell lines. We found that not only the IL-20 is expressed in pancreatic cells but also its receptors are expressed. So we want to evaluate that if IL-20 is a role of autocrine in the pancreas. On the other hand, in H. pylori-infected duodenal ulcer, we found the expression level of IL-20 and IL-20R1 is up regulated, especially in lamina propria cells. And we also found that IL-20 and its functional receptors are expressed in gastric tumor cell lines. However, their implication in clinics remains to be further investigated. These studies could provide us new insights in disease pathogenesis and develop potential therapeutic drugs. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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