Expression of Mitofusins in non-small cell lung cancer
| Autor: | Mei-Yao Lin, 林美瑤 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 The accumulated evidences show that the dynamic behaviors (fission and fusion) of mitochondria are crucial for many cellular functions. Mitochondrial fusion serves to mix and to unify the mitochondrial compartment, which are important in cellular aging, development, energy dissipation and mitochondrial DNA inheritance. Mitofusins, proteins that are expressed on mitochondrial outer membrane, have been identified as key components of mitochondrial fusion machinery in mammalian cells. Moreover, these proteins were shown to mediate cell growth and development. However, the mechanism how mitofusins affect cancer cell growth is still unclear. In this study, we investigated the expression of mitofusins in non-small cell lung cancer. Our results showed that mitofusins were overexpressed in 60% of tumor specimens by RT-PCR, in situ hybridization, Western blot and immunohistochemistry. Besides the predicted hMfn1 (84 kDa), we also detected an extra protein with molecular weight about 100 kDa by Western blot. Although we highly expect that it may be due to a post-translational modification. However, it awaits further determination. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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