The Mechanism of Diosmetin Induced Apoptosis in Human Leukemia Cancer Cell Lines HL-60
| Autor: | Chun-Liang Hu, 胡俊良 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 The purpose of this research is to study the Diosmetin if will cause the apoptosis of cancer cell and the mechanism of the induction of apoptosis by Diosmetin. In our study, Diosmetin has shown its apoptosis-inducing potency in HL-60 with its 49.34% apoptotic ratio of 100μM treatment. When HL-60 were treated with 100μΜ of Diosmetin, DNA ladder were visible at 6 hours and increased from 6-16 hours after treatment. Diosmetin could decrease the mitochondrial membrane potential （ΔΨm）, trigger cytochrome c released to cytosol, and subsequently induced the process of procaspase-9 and procaspase-3, which were followed by the cleavage of PARP and DFF-45. On the other hand, the caspase-8 could be induced and cause the development of truncated Bid and induced cytochrome c released. The hydrogen peroxide and superoxide increase might result oxidative stress increased and induced apoptosis. The cleavage of the proapoptotic Bcl-2 proteins, such as Bad and Bax to produce their truncated forms, and the down-regulation of Bcl-2 were detected in our study. From these results, we suggest that mitochondria might play an important role in the Diosmetin-induced apoptosis. The induction of apoptosis by Diosmetin may offer a pivotal mechanism for its cancer-therapeutic and cancer-preventive effects. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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