A Study on Solid-state Cultivation of Cordyceps militaris

Autor: Chia-hsien Chou, 周佳賢
Rok vydání: 2005
Druh dokumentu: 學位論文 ; thesis
Popis: 93
Cordyceps militaris is an enthomopathogenic fungus that belongs to the class of Ascomycetes. Traditionally, the fruiting bodies of several Cordyceps species have been used as medicine in South East Asian countries as a result of their various physiological activities. The fungus C. militaris is very difficult to collect in nature because it’s shape is very small and its growth is restricted to a specific area. Further, the content of biologically active compound cordycepin in C. militaris is much higher than in C. sinensis. Therefore, mass production of C. militaris is necessarry through artificial cultivation for the commercial exploitation. In recent years solid-state fermentation (SSF) has gained much interest of researchers, as studies have demonstrated superior product yields and simplified downstream processing. The purpose of this study was to optimize the SSF conditions of C. militaris for the production of cordycepin. We find the pupa of silkworm and rice are main materials in solid-state cultivation and simulate natural environment for culturing the fruiting body of C. militaris. The cultivation medium is the rice 32.8 %, PDB 8.2 %, peptone 1.6 %, the pupa of silkworm 7.4 %, the power of soy beans 0.8 % and distilled water 49.2 %. The terms of solid-state cultivation is 20 ℃, relative humidity 70 %, and 1500~2000 lux lighting. After 90 days in solid-state cultivation, the fruiting body of C. militaris will be aquired. The dries fruiting body of C. militaris is extracted by water for cordycepin. Cordycepin is analyzed by HPLC, LC-MS and CE. Respectively, the result of analysis is 45.68 mg/g、34.187mg/g、40.85 mg/g. To display that fruiting body of C. militaris form our laboratory is the quantity of cordycepin more than other fruiting body of C. militaris.
Databáze: Networked Digital Library of Theses & Dissertations
Externí odkaz: