Quantum Dots Surface Modification and Bio-Conjugation
| Autor: | Yiu-Jung Liu, 劉又榕 |
|---|---|
| Rok vydání: | 2005 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 CdSe/ZnS quantum dots (Qdots) were made water soluble by surface modification with the help of mercaptopropionic acid (MPA) or a polymer called Poly(maleic anhydride alt-1-tetradecene). Both methods produced soluble Qdot close to 85% recovery, and the quantum yields were both approaching 50%. The stability of soluble Qdots was also under investigation. It was found that MPA modified dots easily aggregated in 0.1 M NaCl, but the polymer coated dots were fairly stable. The covalently bound bovine serium albumin (BSA) increased the stability of Qdots. We also tried to detect the single mismatched ssDNA by Qdot linked ssDNA. It was found that the hybridization efficiency of single mismatched ssDNA was far lower than the completely complementary one. It indicated that Qdots linked ssDNA could accurately detect mismatched ssDNA. By mean of fluorescence resonance energy transfer (FRET) the number of red fluorescence protein (RFP) absorbed on Qdots could be accurately estimated. There are only one RFP molecule adsorbed on each dot. By adopting Forster’s theory the distance between Qdot and fluorescent center can be calculated by FRET efficiency. Therefore, the orientation of RFP on Qdot could be elucidated. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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