Preparation and Evaluation of a Novel Bone Substitute Composed of Oligomeric Proanthocyanidin Cross-linked Gelatin and Tricalcium Phosphate
| Autor: | Pei-Chi Shyu, 徐佩琪 |
|---|---|
| Rok vydání: | 2006 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 93 The purpose of this study was to prepare and evaluate the feasibility and biocompatibility of a novel composite (GTP) as a large defect bone substitute in-vitro and in-vivo. The composite was combined tricalcium phosphate ceramic particles with oligomeric proanthocyanidin (OPC) cross-linked gelatin. In order to evaluate the crosslinking ability of OPC to gelatin molecules and determine the optimal concentration of OPC solution used in the GTP composite, the fixation index, swelling ratio and in-vitro degradation rate of the novel composite were explored in this work. In addition, substances released from the soaked GTP composites were analyzed after soaking the GTP composites in deionized distilled water at 37℃ for 4, 7, 14, 28 and 42 days. Then, the solution soaking the GTP was co-cultured with MG-63 cell to determine if the released substances from GTP could facilitate the growth of bone cells. Finally, in order to assess the degradable rate in-vivo, biocompatibility of the tissue and the potential for accelerating the reconstruction of defect bone of the composites, the subcutaneous implantation in rats and the rabbit’s cranial bone defect model were used in an in-vivo test. Results indicated that more complete crosslinking reaction in the GTP composite occurred when C wt% of OPC solution had been added. OPC could decrease the degradation rate of gelatin and GTP composites effectively. The results of biocompatibility in vitro showed that the substances released from GTP composites could promote the proliferation and differentiation of MG-63 cell. Finally, the GTP composites were shown a good compatibility during subcutaneous implantation in rats. Radiographic observation of rabbit’s cranial bone defect implanted with GTP composites revealed that the new bone growth into the cranial bone defect progressively. Therefore, the GTP composite could serve as a useful bone substitute for repairing bone defects. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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