Double Integrating Sphere and Monte Carlo Simulation of Cervical Tissue Autofluorescence Spectra
| Autor: | Shi-Hao Hua, 花士豪 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 The goal of the work is to use Monte Carlo (MC) modeling for autofluorescence spectra and clinical measurements of cervical tissues of different dysplasia stages. The optical parameters, absorption and scattering coefficients, which were measured by Double Integrating Sphere system measuring the reflectance and transmittance spectra and estimated by using Inverse Adding-Doubling method. We used MC autofluorescence model to simulate different cervical tissue spectra and predict the autofluorescence spectra, showing different development stages from normal epithelium to cervical intraepithelial neoplasia (CIN). We compared different cervical tissues, including endocervix, endocervix, exocervix and polyp. These results show that the absorption coefficient of exocervix is larger than endocervix and endometrium; a larger scattering coefficient existed in cervical polyp. The experimental spectra show that the cervical spectrum peak at 380 nm (Collagen) is higher than the peak at 450 nm (NADH) and the endocervix spectrum peak at 450 nm is higher than the 380 nm peak. Furthermore, we applied the autofluorescence model to simulate the autofluorescence spectrum of CIN1, CIN2, CIN3 and found that the decreased autofluorescence intensity at 380 nm, 450 nm and 520 nm was accompanied with the increasing thickness of abnormal tissue on the cervical epithelium. These results could provide estimation of autofluorescence characteristics of different dysplasia stages and aid clinical diagnosis in defining the cancer development stages. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|