Functional Characterization of Human Homeobox Gene OTEX
| Autor: | Yi-Fang Wang, 王怡方 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 A pair-like homeobox gene OTEX (Ovary-Testis-Epididymis-homeoboX) was identified through genomic DNA sequence alignment with a previously isolated homeobox gene TSX1. OTEX gene is localized on the human X chromosome and expressed in testis, epididymis, prostate, ovary and brain. No ortholog of OTEX gene has been identified in mouse genome through intensive GenBank search and cDNA library screening. Zoo blot analysis further confirmed that OTEX is only present in human genome. The results suggested that OTEX is appeared in a later period of the evolution, and may be involved in an important genetic regulation of the human physiology. OTEX expression is also identified in both LNCaP and PC3 prostate cancer cell lines, and the expression was stimulated dramatically following androgen treatment. Proscran was used to search binding motif of upstream and intron 1 of OTEX gene, and an ARE site was identified in the upstream region. Since androgen is crucial to the development of prostate cancer, we change the expression level of OTEX in the LNCaP cell lines, to elucidate the potential role of OTEX in prostate cancer. Both transgenic overexpression and RNA interference were induded in our strategy. OTEX was transfected into PC-3 and COS 7 cell lines to understand the effect of OTEX in other cell lines. From the preliminary result, we found that OTEX overexpression seem enhanced the cell proliferation, which imply a correlation between OTEX and prostate cancer. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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