The lipid utilization and egg yield in Taiwan country chicken
| Autor: | Yi-Tien Chen, 陳奕廷 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 In poultry, lipids are mainly synthesized in the liver and transported outward to support body maintenance and reproduction requirements. The superfluous lipids are stored in the adipose or muscle tissues. The objective of this study was to dissect the role of lipid metabolism in the egg production performance, taking advantage of the two Taiwan native chicken lines with relatively high (L2 line) and low (B line) rates of egg production. Samples were collected from chickens at 22 or 50 weeks of age. Expression of the lipid metabolism-related genes were measured by using real-time RT-PCR. In liver, the levels of fatty acid synthetase (FAS), apoprotein B (apo-B), and leptin mRNA’s in the B line were significantly higher than those in the L2 line. In adipose tissue, higher leptin mRNA accumulation levels were seen in the B line than the L2 line. However, the mRNA levels of PPARγ, LPL, VLDLR, and HSL were elevated in the L2 line, compared with those in the B lines. Furthermore, frozen sections were prepared and analyzed to compare the lipid storage in leg muscle and the cell size in adipose tissue. Results showed that the B line chickens have more lipid storage in leg muscle and larger adipocytes in adipose tissue than the L2 line. Taken together, the B line chickens appear to have significant higher capacity in terms of lipid synthesis in liver, as well as transport and storage of lipids in adipose and muscle tissue than the L2 line chickens, suggesting that lipid distribution correlates more to egg production efficiency than lipid synthesis does. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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