Functional roles of CD30 in Hodgkin's disease
| Autor: | Che-Chun Su, 蘇哲俊 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 CD30 is expressed on Hodgkin’s Reed-Sternberg (H-RS) cells, the tumor cells in Hodgkin’s disease (HD). Increased levels of serum CD30 are observed in HD patients and are a good marker to predict a poor prog nosis and responses to therapy. In this study, we addressed the effect of CD30 on T cells by studying the interaction between H-RS cells and T cells. We showed that CD30, either as a membranous protein on H-RS cells and Chinese Hamster Ovary (CHO) cells, or as a pate-bound chimeric protein, inhibited T cell proliferation. Anti-CD3 stimulated T cells in cocultures with H-RS cells failed to increase tritium thymidine uptake, failed to express CD25 and CD26 and produce IL-2. The inhibition of T cell proliferation is however, reversed with addition of exogenous IL-2 or pretreatment of H-RS cells with an antagonistic antibody against CD30. Inability of T cells to express CD25 and CD26 in the cocultures with H-RS cells is in accordance with those results from immunohistochemistry on disease-involved tissues. We conclude that H-RS cells are able to inhibit the proliferation and activation of T cells through the CD30-related interaction. The outcome of this interaction is an ineffective anti-tumor immunity, which is clearly in favor of the growth and survival of the tumor cells. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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