Isolation of a Chromosomal Region Associated with Allantoin Metabolism and Liver Infection in Klebsiella pneumoniae by Microarray
| Autor: | Huei-Chi Chou, 周慧琦 |
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| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 Klebsiella pneumoniae liver abscess with metastatic complications is an emerging infectious disease in Taiwan. To identify genes associated with liver infection, we used a DNA microarray to compare the transcriptional profiles between three strains causing liver abscess and three strains without liver infection. There were 14 clones that revealed higher RNA expression levels in the three liver infection strains and three of these 14 clones contained a region that was absent in MGH 78578. The strain of K. pneumoniae MGH 78578 was isolated from a patient without liver abscess and was chosen for full genome sequencing by the Genomic Sequencing Center at Washington University (St. Louis, MO) (http//genome.wustl.edu/gsc). Sequencing of the clones revealed the replacement of 149 bp of MGH 78578 with a 21,745 bp fragment in a liver infection strain, NTUH-K2044. This 21,745-bp fragment contained 19 open reading frames, 14 of which were proven to be associated with allantoin metabolism. The K2044(�徨llS) mutant revealed a significant decrease of virulence in intragastric inoculation of BALB/c mice and the prevalence rate of this chromosomal region was significantly higher in strains associated with liver abscess than in those that were not (19/32 vs. 2/94, P=.0001, x2 test). Therefore, the 22-kb region may play a role in K. pneumoniae liver infection and serve as a marker for rapid identification. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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