Insecticide Resistance to Pyrethroid in Aedes aegypti
| Autor: | Ying-Hsi Lin, 林鶯熹 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 The resistance ratios of adult mosquitoes of the Chianjen, Lingya (2002), and Lingya (1900R) strains for permethrin compared to susceptible strains were above 312.5X, while those of the 4th instar larvae were 20.8, 37.5, and 102.1, respectively. We used WHO insecticide-impregnated papers to determine the insecticide susceptibility of adult Aedes aegypti in 2002.. The Chianjen and Lingya (2002) strains showed resistance to cyfluthrin. The Chianjen, Lingya (2002), and Shinshing strains showed resistance to deltamethrin. The Chianjen, Lingya (1990R), Lingya (2002), Shinshing, Linyuan and Fengshan strains showed resistance to etofenprox. The Chianjen, Lingya (2002), Shinshing, Sanmin, Tzuoying, Linyuan, Gangshan, Fengshan, Gueiren, Shinshi, S. District, W. District, and C. District strains showed resistance to permethrin. The Sanmin, Tzuoying, and Gangshan strains showed resistance to lambdacyhalothrin. 2003, the Chianjen, Linya and Fengshan strains of Aedes aegypti were resistant to both etofenprox and permethrin, but the Chianjen and Lingya (2002) reduced resistance to cyfluthrin and deltamethrin. The Tainan South District strain of Aedes aegypti showed the resistant to permethrin and cyhalothrin. The Tainan Gueiren strain of Aedes aegypti showed the resistant to permethrin and cyhalothrin. Results of a synergism study on larvae resistant to permethrin showed correlations to the activities of microsomal monooxygenases and esterases. The highest specific activities of esterase are 0.249�b0.011 and 0.248�b0.009 m mole/min/mg protein in Chianjen strain adults with ?naphthyl acetate andβ-naphthyl acetate, respectively. The specific activities of esterase in larvae are not related with resistance when using ?naphthyl acetate or β-naphthyl acetate as substrate. We cloning of cytochrome P450 from Ae. aegypti resistant to permethrin, and amplified a partial cDNA fragment about 250bp fragment.in RT-PCR. Southern hybridization using a CYP6F1 cDNA fragment as a probe showed a signal. We got six partial P450 sequence, one was 249bp and the other five were 231bp. W103 was low similarity with the others from 63.1% to 66.2%. W103 was higher similarities with the sequences of dipterean that has known than the other five sequences, 45.1-59.0%. The highest similarity with CYP6P5 from Anopheles minimus, 59.0%. There were 6 bands using semi-quantitative RT-PCR analysis, 1.4kb, 750bp, 600bp, 350bp, 300bp, and 250bp. 300bp fragment transcript was greater in the permethrin-resistant Ae. aegypti compared to the susceptible strain. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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