The study of acute toxicity and induced metallothioneins by cadmium in mosquitofish, Gambusia affinis
| Autor: | Hui-Yan Wang, 王慧燕 |
|---|---|
| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 Until September 2001, the cadmium-rice matter was occurred in Taiwan. The half-life of cadmium keeps for 20 years. Obviously, pollution of cadmium can’t be neglected any more, and the monitor of cadmium pollution can’t be idled. Therefore, this study chooses Gambusia affinis, which lives in rice field, to proceed the experiments of Cd acute toxicity and Cd-induced metallothionein. In order to confer the variation of Cd-induced metallothioneins in different soak duration, first, Bradford method and UV-visible spectrophotometer were employed to measure the protein consistency of Gambusia affinis sample. Second, SBD-F fluorescent method was used to measure the metallothioneins of Gambusia affinis sample. Resultly, in the acute toxicity experiment, a master lethal curves of Gambusia affinis with cadmium solution, S = 1/{1+exp[5.7308-0.0784(T+8.6)/(C-2.63)], was obtained. In the Cd-induced metallothionein experiment, soaked in lower cadmium (0.25 ppm), the Cd-induced metallothionein (MTs) increases continuously to the maximum quantity（1140.7 ± 168.7μg/g）in 16 days. But, soaked in higher cadmium (1 ppm), the Cd-induced metallothionein increases quickly. The MTs quantity does not increase when reach a fixed consistency. And the MTs increase instantly but not progressively, even reduce in 0.5 and 0.75 ppm Cd solution. It shows metallothioneins have a fixed carry capacity to combine cadmium. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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