Characterization of a Cysteine-rich Venom Protein from Trimeresurus mucrosquamatus (Taiwan habu)
| Autor: | Chung-Li Ho, 何宗澧 |
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| Rok vydání: | 2004 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 92 The cDNA encoding cysteine-rich venom protein (CRVP) was isolated from a cDNA library of venom gland of Trimeresurus mucrosquamatus. CRVP was belonged to the family of cysteine-rich secretory proteins, CRISPs. The CRISPs have sixteen consensus cysteines in their C terminus which can be bridged to form eight disulfide bonds. There were several snake venom proteins belonging to the CRISPs been isolated, such as ablomin, pseudechetoxin, tigrin and so on. CRISPs displayed a specific commom activity in the inhibition of ion channels. When recombinant CRVP was given to mice, a Ca2+ channel blocker resemblance effects of lethargy, paralysis, hypothermia, and death were observed. In addition, we also found that CRVP decreased the blood pressure of the rats without disturbing their heart beats. Therefore, CRVP may be ideally for the development of hypotensive drugs. In this study, CRVP could led into hypothermia and htpotension following intravenous administration in vivo. However, there were no similar effects in vivo in intracereventricular administration. In the search of possible biological mechanisms, we have found that CRVP could degrade kininogen to produce bradykinin in vitro. It is similar to some snake venom serine protease that produces it’s hypotensive effect by kallikrain-like protease activities. In this study, we successfully produced a functional CRVP from a yeast expression system and found that the hypotensive effect of CRVP might be mediated through kininogenase activity. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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